序列：MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG
Ub-Rho110 is a fluorescence probe for Deubiqutinase.Cleavage at the ubiquitin-rhodamine linkage resulted in an intensive fluorescence signal when excited at 485 nm with a high dynamic range. Since this assay is highly sensitive, substrate turnovers of a few hundred femtomoles can easily be measured, and the assay can thus be applied for the screening and profiling of compounds in a high-throughput manner.
